Fig. 4.

Overlap between phosphosites that respond to AZD or PLX. A, Log₂ values of the median SILAC ratios for all phosphosites identified in at least two replicate experiments. Histograms are plotted for AZD/DMSO, PLX/DMSO, and PLX/AZD ratios. B, Area-proportional Venn diagrams showing the overlap in phosphosites with significant AZD/DMSO or PLX/DMSO ratios based on median values. Diagrams distinguish between phosphosites that increase or decrease in response to inhibitor and between phosphosites that do or do not contain the minimal ERK1/2 phosphorylation motif (Ser/Thr-Pro). C, Scatter plot of the log₂ values of the median AZD/DMSO and PLX/DMSO ratios for individual phosphosites. Colors reflect phosphosites that change significantly with both AZD and PLX (cyan), either AZD or PLX (green), or neither inhibitor (gray), using a significance threshold of log₂ value ±0.840. Many phosphosites with apparent responses to only one inhibitor actually change with both, but narrowly miss the significance cutoff with the other inhibitor. Dashed box indicates the significance threshold.