Table I. Detection and quantification results obtained for triplicated IS-PRM and regular PRM (PRM-method A and PRM-method B) analyses of 606 pairs of SIL and endogenous peptides in a plasma sample with associated acquisition parameters.

Method	Acquisition parameters				Number of peptides systematically identifieda	Number of peptides reliably quantifiedb
	SIL peptides		Endogenous peptides
	Orbitrap resolving powerc	Fill time	Orbitrap resolving powerc	Fill time
IS-PRM	15000	20 ms	60000	110 ms	251	251
PRM-Method A	60000	110 ms	60000	110 ms	203	124
PRM-Method B	15000	20 ms	15000	20 ms	129	129

^a Endogenous peptides in triplicated analyses of plasma sample.

^b Coefficient of variation below 20%.

^c High field orbitrap analyzer at m/z 200.