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The two panels show the MALDI-TOF-MS spectra from two adjacent spots (n and n+1 s). In the treated spot at Nr 608 (= spot n), the deglycosylated peptide of Asn 332 can be detected. This peak is absent in the consecutive spot Nr 609 (= spot n+1), which is untreated. The untreated spots contain the intact glycopeptides and were further analyzed with a high resolution MALDI-FTMS instrument (Fig. 5, and supplemental Fig. S3). (All peaks are labeled with monoisotopic masses).
