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. 2015 Mar 23;14(6):1645–1656. doi: 10.1074/mcp.O114.046748

Table II. Identified glycopeptide peaks and corresponding glycan compositions obtained by analyzing the peak lists from the FTMS spectra with GlycoMod tool (mass deviations were averaged over acquired spectra). An absolute mass deviation below or equal 0.025 Da (in average if the structure was identified in more than one spectrum) was set as limit. (Greater deviations were accepted for some lower intense peaks whose corresponding spectra were further investigated manually).

Nr. Asn Glycopep. [M+H]+ calculated Deviation ppm (av) Deviation Da (av) Glycan (mi) calc. Composition Type Peptide (mi) calc Sequence
calc.
1 171 3838.5280 −0.8 −0.003 2553.91 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
2 171 3635.4480 −2.2 −0.008 2350.83 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
3 171 3547.4320 0.0 0.000 2262.81 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
4 171 3401.3740 −6.8 −0.023 2116.76 (Hex)2 (HexNAc)3 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
5 171 3344.3530 0.3 0.001 2059.74 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
6 171 3198.2950 −1.3 −0.004 1913.68 (Hex)2 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
7 171 3256.3370 −7.7 −0.025 1971.72 (Hex)2 (HexNAc)3 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
8 171 3053.2580 −4.9 −0.015 1768.64 (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
9 171 2907.137 −20.0 >0.025 1913.68 (Hex)2 (HexNAc)2 + (Man)3(GlcNAc)2 Complex 1283.61 YKNNSDISSTR (1 missed cleavage)
10 171 3303.326 5.8 0.019 2018.71 (Hex)3 (HexNAc)1 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Hybrid 1283.61 YKNNSDISSTR (1 missed cleavage)
11 171 3141.2740 2.2 0.007 1856.66 (Hex)2 (HexNAc)1 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Hybrid 1283.61 YKNNSDISSTR (1 missed cleavage)
12 171 2979.2210 −0.3 −0.001 1694.60 (Hex)1 (HexNAc)1 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Hybrid/complex 1283.61 YKNNSDISSTR (1 missed cleavage)
13 171 3012.2310 −3.3 −0.010 1727.61 (Hex)3 (HexNAc)1 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Hybrid 1283.61 YKNNSDISSTR (1 missed cleavage)
14 171 2850.1780 9.5 >0.025 1565.56 (Hex)2 (HexNAc)1 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Hybrid 1283.61 YKNNSDISSTR (1 missed cleavage)
15 171 2688.1250 −1.1 −0.003 1403.51 (Hex)1 (HexNAc)1 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Hybrid/complex 1283.61 YKNNSDISSTR (1 missed cleavage)
16 171 2501.0410 1.6 0.004 1216.42 (Hex)2 + (Man)3(GlcNAc)2 Oligomannosidic 1283.61 YKNNSDISSTR (1 missed cleavage)
17 171 2663.0940 −4.1 −0.011 1378.48 (Hex)3 + (Man)3(GlcNAc)2 Oligomannosidic 1283.61 YKNNSDISSTR (1 missed cleavage)
18 171 3547.3690 19.7 >0.025 2553.91 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
19 171 3344.2890 19.7 >0.025 2350.83 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
20 171 3256.3370 10.0 >0.025 2262.81 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
21 171 3110.216 10.1 >0.025 2262.81 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
22 171 3053.195 9.8 >0.025 2059.74 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
23 171 2907.137 1.4 0.004 1913.68 (Hex)2 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
24 171 2762.1 2.0 0.006 1768.64 (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
25 171 2616.042 −5.0 −0.013 1622.58 (Hex)2 (HexNAc)2 + (Man)3(GlcNAc)2 Complex 992.45 NNSDISSTR
26 171 2371.936 −6.3 −0.015 1378.48 (Hex)3 + (Man)3(GlcNAc)2 Oligomannosidic 992.45 NNSDISSTR
27 171 2209.883 −9.5 −0.021 1216.42 (Hex)2 + (Man)3(GlcNAc)2 Oligomannosidic 992.45 NNSDISSTR
28 395 3355.283 −1.4 −0.005 2553.91 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
29 395 3152.203 −0.6 −0.007 2350.83 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
30 395 3064.188 −1.3 −0.005 2262.814 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
31 395 3209.226 −0.5 −0.003 2407.852 (Hex)2 (HexNAc)3 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
32 395 2699.056 −0.9 −0.004 1897.682 (Hex)1 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
33 395 2861.109 −1.7 −0.006 2059.735 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
34 395 2918.13 −2.5 −0.009 2116.756 (Hex)2 (HexNAc)3 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
35 395 2902.136 −0.9 −0.008 2100.762 (Hex)1 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 800.37 THTNISE
36 402 2270.929 −1.8 −0.004 1054.37 (Hex)4 (HexNAc)2 Paucimannosidic 1215.55 SHPNATFSAVGE
37 402 2432.982 −0.4 −0.001 1216.423 (Hex)2 + (Man)3(GlcNAc)2 Oligomannosidic 1215.55 SHPNATFSAVGE
38 402 2595.035 0.0 0.000 1378.476 (Hex)3 + (Man)3(GlcNAc)2 Oligomannosidic 1215.55 SHPNATFSAVGE
39 402 2757.087 −0.7 −0.002 1540.528 (Hex)4 + (Man)3(GlcNAc)2 Oligomannosidic 1215.55 SHPNATFSAVGE
40 402 2919.14 −0.7 −0.002 1702.581 (Hex)5 + (Man)3(GlcNAc)2 Oligomannosidic 1215.55 SHPNATFSAVGE
41 402 3081.193 −2.6 −0.008 1864.634 (Hex)6 + (Man)3(GlcNAc)2 Oligomannosidic 1215.55 SHPNATFSAVGE
42 402 2311.956 −0.9 −0.002 1095.397 (HexNAc)1 + (Man)3(GlcNAc)2 Hybrid/complex 1215.55 SHPNATFSAVGE
43 402 2474.008 −4.9 −0.012 1257.449 (Hex)1 (HexNAc)1 + (Man)3(GlcNAc)2 Hybrid/complex 1215.55 SHPNATFSAVGE
44 402 2636.061 −2.3 −0.006 1419.502 (Hex)2 (HexNAc)1 + (Man)3(GlcNAc)2 Hybrid/complex 1215.55 SHPNATFSAVGE
45 402 2798.114 −3.2 −0.009 1581.555 (Hex)3 (HexNAc)1 + (Man)3(GlcNAc)2 Hybrid 1215.55 SHPNATFSAVGE
46 332 4908.98 ∼75 >0.025 2553.91 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
47 332 4705.9 −8.0 >0.025 2350.83 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
48 332 4617.884 0.9 0.004 2262.814 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
49 332 4471.826 0.0 0.000 2116.756 (Hex)2 (HexNAc)3 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
50 332 4455.832 4.0 0.018 2100.762 (Hex)1 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
51 332 4414.805 4.3 0.019 2059.735 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
52 332 4326.789 3.7 0.016 1971.719 (Hex)2 (HexNAc)3 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
53 332 4268.747 3.7 0.016 1913.677 (Hex)2 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
54 332 4123.71 2.2 0.009 1768.64 (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
55 332 4430.8 4.3 0.019 2075.73 (Hex)3 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2 Hybrid 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
56 332 4164.736 3.8 0.016 1809.666 (Hex)1 (HexNAc)3 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 2354.06 GLTFQQNASSM(SO)CVPDQDTAIR
57 332 4892.982 ∼50 >0.025 2553.91 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
58 332 4689.905 −1.5 −0.007 2350.83 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)2 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
59 332 4601.889 3.5 0.016 2262.814 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
60 332 4455.831 4.0 0.018 2116.756 (Hex)2 (HexNAc)3 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
61 332 4439.837 4.1 0.018 2100.762 (Hex)1 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
62 332 4398.81 4.1 0.018 2059.735 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
63 332 4310.794 3.0 0.013 1971.719 (Hex)2 (HexNAc)3 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
64 332 4252.752 6.6 >0.025 1913.677 (Hex)2 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
65 332 4107.715 6.6 −0.002 1768.64 (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
66 332 4414.805 4.3 0.019 2075.73 (Hex)3 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2 Hybrid 2338.07 GLTFQQNASSMCVPDQDTAIR
67 332 4148.741 1.9 0.008 1809.666 (Hex)1 (HexNAc)3 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 2338.07 GLTFQQNASSMCVPDQDTAIR
68 563 3435.47 5.5 0.019 1054.37 (Hex)4 (HexNAc)2 Paucimannosidic 2380.09 STGKPTLYNVSLV(MSO)SDTAGTCY
69 563 3597.523 3.3 0.012 1216.423 (Hex)2 + (Man)3(GlcNAc)2 Oligomannosidic 2380.09 STGKPTLYNVSLV(MSO)SDTAGTCY
70 563 3759.576 2.9 0.011 1378.476 (Hex)3 + (Man)3(GlcNAc)2 Oligomannosidic 2380.09 STGKPTLYNVSLV(MSO)SDTAGTCY
71 563 3921.628 2.3 0.009 1540.528 (Hex)4 + (Man)3(GlcNAc)2 Oligomannosidic 2380.09 STGKPTLYNVSLV(MSO)SDTAGTCY
72 563 4083.681 2.2 0.009 1702.581 (Hex)5 + (Man)3(GlcNAc)2 Oligomannosidic 2380.09 STGKPTLYNVSLV(MSO)SDTAGTCY
73 563 4245.734 5.9 0.025 1864.634 (Hex)6 + (Man)3(GlcNAc)2 Oligomannosidic 2380.09 STGKPTLYNVSLV(MSO)SDTAGTCY
74 71 (J-chain) 3142.294 0.8 0.003 1913.677 (Hex)2 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR
75 71 (J-chain) 3288.352 −0.3 −0.001 2059.735 (Hex)2 (HexNAc)2 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR
76 71 (J-chain) 3345.373 2.4 0.008 2116.756 (Hex)2 (HexNAc)3 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR
77 71 (J-chain) 2851.199 2.5 0.007 1622.582 (Hex)2 (HexNAc)2 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR
78 71 (J-chain) 3491.431 0.7 0.002 2262.814 (Hex)2 (HexNAc)3 (Deoxyhexose)1 (NeuAc)1 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR
79 71 (J-chain) 2997.257 −1.6 −0.005 1768.64 (Hex)2 (HexNAc)2 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR
80 71 (J-chain) 3054.278 0.5 0.002 1825.661 (Hex)2 (HexNAc)3 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR
81 71 (J-chain) 3200.34 −2.2 −0.007 1826.661 (Hex)2 (HexNAc)3 (Deoxyhexose)1 + (Man)3(GlcNAc)2 Complex 1227.61 ENISDPTSPLR