Fig. 4.

PUC-ERLIC facilitates enrichment of the N-glycoproteome of human plasma. A, Proportion of unique N-glycoproteins, N-glycopeptides, and N-glycosylated sites obtained using PUC-ERLIC, PUC alone, or ERLIC alone (all with prior PNGase F treatment). The glycosylation percentage was calculated using the number of unique glycosylated products divided by the sum of all unique glycosylated and unmodified peptides. B, Venn diagram of overlapping plasma N-glycoproteins obtained using PUC-ERLIC, PUC alone, or ERLIC alone.