Binding of CLL mAbs assigned to stereotyped subsets #1, #2, and #8 to TLR and nod-like receptor ligands. CLL mAbs were used as primary Ab for the colorimetric ELISA. Samples were grouped on the basis of BCR stereotypy. The bars represent the mean OD value as measured for CLL mAbs assigned to each of the stereotyped subsets #1, #2, or #8. The mAbs tested and the mean OD values of 3 replicates per sample are listed in supplemental Table 5. All determinations were performed in triplicate. *P < .05; **P < .005; ***P < .001. LPS, lipopolysaccharides; MPD, muramyldipeptide.