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Summary of the Cryptosporidium spp. and Giardia lamblia detection method optimization. The selected options are highlighted with gray. Quantitative polymerase chain reaction (qPCR) primer and probe concentrations were tested separately for both parasites and combined into a multiplex reaction. Stool kit (w/o iEx) = stool kit without the inhibitEx tablet included in the kit; Fwd = forward (primer); Rev = reverse (primer).
