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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Reproduction. 2015 Apr 1;149(6):657–663. doi: 10.1530/REP-15-0017

Figure 6.

Figure 6

Spermatozoa with both patterns of EGFP distribution could undergo the AR after the addition of progesterone. (A) Time-lapse images of EGFP-expressing spermatozoa with the AC pattern (upper panels) or the EQ pattern (lower panels) undergoing acrosomal exocytosis after the addition of 20 μM of progesterone. (B) The percentages of ARs of both AC- and EQ-type spermatozoa in the presence (induced AR) or absence (spontaneous AR) of 20 μM of progesterone. As a control, the vehicle DMSO was added to the samples (Medium). Spermatozoa were attached to laminin-coated slides and recorded for 10 min. During this time, both patterns underwent spontaneous ARs at similar rates. However, in the presence of 20 μM progesterone, more EQ sperm tended to undergo exocytosis than the AC sperm. Data represent the mean ± SEM (n = 4 experiments). *represents significant difference compared with AC pattern (P< 0.05).