Figure 5. Importance of C328 in the response of the vimentin network to electrophilic and oxidative stress.

SW13 cells stably transfected with RFP//vimentin wt or C328S were treated with 10 μM HNE for 4 h, 30 μM PGA₁ for 20 h or 1 mM diamide for 20 min. (a) Vimentin network visualized by IF. Insets depict cytoplasmic RFP fluorescence and 4,6-diamidino-2-phenylindole nuclear staining; n≥3. (b) IF of tubulin. Insets show vimentin distribution and zoom-ins of areas of interest. Scale bars, 20 μm.