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. 2015 May 28;6:7203. doi: 10.1038/ncomms8203

Figure 3. RUNX1-ETO induction disturbs differentiation-dependent gene expression changes during the EHT.

Figure 3

(a) Heat map representing the fold change of endothelial gene expression between RUNX1-ETO-induced and uninduced cultures in the HE and HE2. Each line represents the RNA fold change in expression of individual genes between the induced and uninduced cultures. Red and blue indicate increased and decreased expression, respectively. (b) RUNX1-ETO expression regulates pre- and post-RUNX1 transcription factors genes differently. Heat map representing changes in expression of haematopoietic transcription factors between induced and uninduced cultures in the HE and HE2. Each line represents the RNA fold change in expression of individual genes between the induced and uninduced cultures. (c) PCR with reverse transcription validation of microarray results using RNA isolated from HE and HE2 of the RUNX1-ETO-induced and uninduced cultures. Error bars represent the standard error of three independent experiments.