Figure 7. Effect of 1-butanol on cortical actin array remodelling following MAMP treatments.

Percentage of occupancy or density was measured on epidermal cells of WT and cpb-1 mutants treated with 0, 0.5% 1-butanol or 1% 2-butanol for 15 min, and followed by 5-min treatment with 1 μM elf26 (a) or 1 μM chitin (c). Treatments with 1-butanol significantly decreased the density of actin array in WT cells, as shown previously29. The actin abundance in WT cells treated with 1-butanol failed to increase following MAMP treatments. By contrast, actin filament abundance in the cpb-1 mutants was not altered by either treatment. Treatments with 2-butanol had no measurable effects on actin filament levels in either WT or cpb-1 seedlings. No significant differences in the extent of filament bundling were observed in WT and cpb-1 mutant cells among treatments (b,d). Values given are mean±s.e.m. (n⩾200 cells from 10 hypocotyls for each treatment and genotype; ***P<0.001; ND, no significant difference compared with mock control of the same genotype; Student's t-test).