Figure 8.

Leptin-induced NF-κB pathway activation is not mediated by stimulation of the leptin receptor. Cells were treated with leptin 50 ng·mL⁻¹ for 4 and 50 min in the presence or absence of SHLA (100 ng·mL⁻¹). (A) Western blots for p-NF-κB. The blots are representative of four independent experiments. Quantification: 65 kD p-NF-κB bands were digitized and quantified with QuantityOne. Phosphorylated bands are compared with total of bands formed for each condition and presented as mean values ± SEM. (B) P-NF-κB immunofluorescence: cells were labelled with anti-P-NF-κB antibody, Alexa fluor 488 and with DAPI for nuclear localization. Pictures, taken with an epifluorescence microscope (×1000) in random chosen fields, are representative of five pictures taken for each condition. Quantification: bars represent the mean ± SEM from three independent experiments.