Figure 5.
The effect of OEA over time on BBB permeability in the initial endocannabinoid screening (A) (n = 6 inserts from three separate experiments) as a concentration-response curve (B) (n = 4–6 inserts from three separate experiments) and in the presence of the PPARα antagonist GW6471 (C and D) (n = 5 inserts from three separate experiments). (E) The effects of NADA (10 μM) on TEER values in the BBB model (n = 7–8 inserts from four separate experiments). (F) Absorbance values for LDH assay conducted on cell culture medium obtained from the luminal (endothelial) chamber of the inserts at 48 h (n = 6 inserts from three separate experiments). Data are given as mean ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05; OEA compared to vehicles treated inserts; †††P < 0.001, ††P < 0.01; OEA and antagonist compared with OEA alone; one-way anova with Dunnett's or Bonferroni's test.