Figure 5.

PGE₂ and TXA₂ induce cell migration. (A,B) Wound healing assays performed in rat VSMCs unstimulated and stimulated with different concentrations of U46619 or PGE₂. (C) Transwell migration assay of VSMCs unstimulated and stimulated with U46619 or PGE₂. (D,E) Wound healing assays performed in rat VSMCs unstimulated and stimulated with U46619 or PGE₂ with or without inhibitors of TP receptor (SQ29548, SQ), EP1 receptor (SC19220, SC), EP3 receptor (L798106, L7), ERK1/2 (U0126, U), JNK (SP600125, SP), p38 MAPK (SB203580, SB), PI3K (LY294002, LY), PKC (chelerythrine, Ch), CaM (W7, W12), pCaMKII (KN93, KN) or EGFR (AG1478, AG). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus control; ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001 versus AngII + IL-1β. n = 3–6.