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. 2015 Apr 10;172(12):3159–3176. doi: 10.1111/bph.13117

Figure 7.

Figure 7

AngII activates MyD88, JNK and NF-κB in VSMCs. (A) Effect of ST-2825 (20 μM, 1 h) on AngII (100 nM, 1 h)-induced NADPH oxidase activity. (B) Effect of CLI-095 (1 μM, 1 h) on AngII (10 min)-induced p-JNK1/2 protein expression; a representative blot is shown above. (C) Effect of CLI-095 on nuclear p65 NF-κB protein expression induced by AngII (45 min); representative blots of the cytosolic (Cy) and nuclear (Nu) expression are shown above; Histone-3 (H3) expressions are also shown to validate the cellular fractioning. (D) Representative photomicrographs of p65 NF-κB immunofluorescence (red) in VSMC in control and after incubation with AngII in the absence and the presence of CLI-095. The insets are magnified images of indicated areas. Images were captured with a fluorescence microscope (×20 objective); image size: 444.16 × 332.8 μm. *P < 0.05 versus control, #P < 0.05 versus AngII. n = 4–5.