Table 1. Antibodies used for immunohistochemistry.
| Antigen | Host | Cat no. | Source | Dilution |
|---|---|---|---|---|
| Primary | ||||
| Calbindin | Rabbit | CB-38a | Swant | 1:2000 |
| CGRP | Goat | ab36001 | Abcam | 1:200 |
| ChAT | Goat | AB144P | Millipore | 1:100 |
| Chx10 | Sheep | AB9016 | Millipore | 1:400 |
| CTB | Goat | 703 | List | 1:100 |
| DCX | Rabbit | ab18723 | Abcam | 1:1000 |
| GFAP | Rabbit | 2016-04 | DAKO | 1:500 |
| GFAP | Mouse | MAB3402 | Millipore | 1:200 |
| GFP | Mouse | A11120 | Molecular Probes | 1:100 |
| Hsp27 | Rabbit | SPA-803 | Nordic Bioscience | 1:300 |
| HuC/D | Mouse | A21271 | Novex (Life Technologies) | 1:400 |
| HuNu | Mouse | MAB1281 | Chemicon | 1:50 |
| IB4 | Goat | AS-2104 | List | 1:100 |
| Islet1 | Mouse | 40.2D6 | DSHB | 1:200 |
| Laminin | Rabbit | L9393 | Sigma | 1:50 |
| Map2 | Chicken | ab5392 | Abcam | 1:1500 |
| Msx1 + 2 | Mouse | AB 531788 | DSHB | 1:50 |
| Nkx6.1 | Mouse | F55A10 | DSHB | 1:20 |
| Pax7 | Mouse | PAX7 | DSHB | 1:50 |
| RT97 (NF200) | Mouse | 1178709 | Mannheim Boehringer | 1:50 |
| Stem123 (huGFAP) | Mouse | AB-123-U-050 | StemCells | 1:1000 |
| TH | Rabbit | P40101 | Pel-Freez | 1:100 |
| VGluT2 | Guinea Pig | AB2251 | Millipore | 1:5000 |
| VIAAT | Rabbit | 131 002 | Synaptic Systems | 1:100 |
| Secondary | ||||
| Alexa 350 | Mouse | A10035 | Invitrogen | 1:500 |
| Alexa 488 | Mouse | A21202 | Invitrogen | 1:1000 |
| Alexa 488 | Rabbit | A11008 | Invitrogen | 1:1000 |
| Alexa 546 | Chicken | A11040 | Invitrogen | 1:1000 |
| Alexa 555 | Goat | A21432 | Invitrogen | 1:1000 |
| Alexa 555 | Rabbit | A31572 | Invitrogen | 1:1000 |
| Alexa 647 | Mouse | A31571 | Invitrogen | 1:1000 |
| Cy3 | Guinea Pig | 706-165-148 | Jackson | 1:1000 |
| Cy3 | Mouse | 715-165-151 | Jackson | 1:1000 |