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. 2015 Jun 8;5:130. doi: 10.3389/fonc.2015.00130

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Copyright © 2015 Ridzewski, Rettberg, Dittmann, Cuvelier, Fulda and Hahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of Annexin V/PI positivity of RD, RMS-13 and Rh41 cells after treatment with GDC-0449, LDE225, HhA, and cyclopamine (cp) at the concentration indicated. Apoptosis was analyzed by FACS after staining of the cells with Annexin V and Propidium Iodide (PI). Vital cells are Annexin V⁻/PI⁻, early-apoptotic (Annexin V⁺/PI⁻) and late-apoptotic cells (Annexin V⁺/PI⁺) were summarized as Annexin V⁺ cells and Annexin V⁻/PI⁺ are necrotic cells, respectively. All data represent at least three independent experiments measured in duplicates. The data are represented as mean ± SEM; solv, solvent.