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. 2015 Jun 8;6:284. doi: 10.3389/fimmu.2015.00284

Figure 3.

Figure 3

Receptors involved in the recognition of bladder cancer cells by IL-2-activated NK cells. (A) The effect of activating receptor blockade was studied in degranulation experiments measuring surface LAMP-1 (CD107a) by flow cytometry. NK cells were pre-incubated with antibodies against the indicated receptors before the co-incubation with bladder cancer targets for 2 h at an E:T ratio of 1:2. (B) The effect of NKG2D blockade was also analyzed in cytotoxicity experiments measuring specific calcein release from bladder target cells. Target cells were labeled with calcein for 16 h, washed and incubated for 4 h with NK cells at an E:T ratio of 10:1. Specific calcein release was measured in supernatants and the percentage of cytotoxicity calculated. (C) The effect of MHC blockade was studied in degranulation experiments measuring surface LAMP-1 by flow cytometry. Each panel shows values of several independent experiments for each cell line.