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. 2015 Jun 8;5:11128. doi: 10.1038/srep11128

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(a) Plasmid architecture. To produce 3-methyl-2-buten-1-ol and 3-methyl-1-butanol in addition to 3-methyl-3-buten-1-ol, plasmid 1 from strain KG1 (Cm^R, p15A ori) was paired with a mixed alcohol production plasmid (Amp^R, pBR322 ori) encoding an Idi1~NudB fusion protein and a reductase (NemA)¹² to create strain KG8. (b) Mixed alcohol production in strain KG8. A fermentation time-course revealed that strain KG8 produced three C₅ alcohols. By 48 hours, strain KG8 produced over 400 mg/L of 3-methyl-1-buten-1-ol, 150 mg/L of 3-methyl-2-buten-1-ol, and 100 mg/L of 3-methyl-1-butanol. All glucose was consumed by 36 hours, and acetate secretion was minimal. Error bars represent standard deviation (n = 3).