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. 2015 Feb 3;5:2. doi: 10.1186/2045-3701-5-2

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© Kim et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Evaluation of cytotoxicity by radiation. MDA-MB-231 cells were treated with the indicated dose of radiation and incubated for 24, 48, or 72 h. Cell viability was determined by trypan blue exclusion assay (A), MTT assay (B), and FACS analysis using propidium iodide (PI) fluorescent dye (C). (D) MDA-MB-231 cells were treated with a single dose of radiation (10 Gy) or fractionated dose of radiation (2 Gy per day for 5 days). Viable cells were determined by trypan blue exclusion assay 24, 48, or 72 h after final irradiation.