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. 2015 Feb 3;5:2. doi: 10.1186/2045-3701-5-2

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© Kim et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Venn diagram summary of identified proteins by SILAC-based quantitative proteomics. (A) Schematic workflow for profiling radiation-induced proteins via proteomic-based analysis. (B) The cytoplasmic lysates from SILAC-labeled cells were analyzed by western blotting to exclude contamination of nuclear extracts using GAPDH and Lamin A/C as cytosolic and nuclear control proteins, respectively. (C) 734 proteins were identified in the set of single dose experiments, and (D) 607 proteins were identified in the set of fractionated dose experiments. (E) Comparison of identified proteins from single or fractionated dose of irradiation.