Table 1.
Up-regulated proteins were identified by a SILAC-based proteomic approach
| no | Accession No | Gene symbol | Identified proteins | Fold | P value * | S 1 | S 2 | F 1 | F 2 |
|---|---|---|---|---|---|---|---|---|---|
| Commonly up-regulated proteins | |||||||||
| 1 | IPI00022418 | FN1 | Isoform 1 of Fibronectin | 6.525 | 0.0000 | 1.7 | 1.9 | 17.7 | 4.8 |
| 2 | IPI00011229 | CTSD | Cathepsin D | 3.200 | 0.0000 | 2.9 | 3.2 | 3.4 | 3.3 |
| 3 | IPI00026314 | GSN | Isoform 1 of Gelsolin | 2.375 | 0.0000 | 3 | 2.6 | 2 | 1.9 |
| 4 | IPI00020632 | ASS1 | Argininosuccinate synthase | 2.175 | 0.0007 | 2.3 | 2.1 | 2.4 | 1.9 |
| 5 | IPI00024915 | PRDX5 | Isoform Mitochondrial of Peroxiredoxin-5, mitochondrial | 2.025 | 0.0606 | 2.9 | 1.8 | 1.5 | 1.9 |
| 6 | IPI00019157 | CSPG4 | Chondroitin sulfate proteoglycan 4 | 1.900 | 0.3173 | 1.4 | 1 | 2 | 3.2 |
| 7 | IPI00218474 | ENO3 | Isoform 1 of Beta-enolase | 1.900 | 0.0145 | 1 | 1 | 4.6 | 1 |
| 8 | IPI00005707 | MRC2 | C-type mannose receptor 2 | 1.850 | 0.0074 | 1.3 | 1.2 | 2.5 | 2.4 |
| 9 | IPI00940829 | NEDD4 | Isoform 4 of E3 ubiquitin-protein ligase NEDD4 | 1.850 | 0.0004 | 1.3 | 1.3 | 2.4 | 2.4 |
| 10 | IPI00021048 | MYOF | Isoform 1 of Myoferlin | 1.800 | 0.0000 | 1.8 | 1.7 | 2.4 | 1.3 |
| 11 | IPI00021812 | AHNAK | Neuroblast differentiation-associated protein AHNAK | 1.800 | 0.0047 | 1.8 | 2.2 | 1.6 | 1.6 |
| 12 | IPI00020557 | LRP1 | Prolow-density lipoprotein receptor-related protein 1 | 1.800 | 0.0000 | 1.4 | 1.4 | 1.8 | 2.6 |
| 13 | IPI00021766 | RTN4 | Isoform 1 of Reticulon-4 | 1.725 | 0.0019 | 1.6 | 1.5 | 1.7 | 2.1 |
| 14 | IPI00006663 | ALDH2 | Aldehyde dehydrogenase, mitochondrial | 1.625 | 0.0262 | 2.1 | 1.8 | 1.4 | 1.2 |
| Single (10 Gy) | |||||||||
| 1 | IPI00011229 | CTSD | Cathepsin D | 3.05 | 2.9 | 3.2 | |||
| 2 | IPI00026314 | GSN | Isoform 1 of Gelsolin | 2.8 | 3 | 2.6 | |||
| 3 | IPI00024915 | PRDX5 | Isoform Mitochondrial of Peroxiredoxin-5, mitochondrial | 2.35 | 2.9 | 1.8 | |||
| 4 | IPI00020632 | ASS1 | Argininosuccinate synthase | 2.2 | 2.3 | 2.1 | |||
| 5 | IPI00021812 | AHNAK | Neuroblast differentiation-associated protein AHNAK | 2 | 1.8 | 2.2 | |||
| 6 | IPI00006663 | ALDH2 | Aldehyde dehydrogenase, mitochondrial | 1.95 | 2.1 | 1.8 | |||
| 7 | IPI00022418 | FN1 | Isoform 1 of Fibronectin | 1.8 | 1.7 | 1.9 | |||
| 8 | IPI00021048 | MYOF | Isoform 1 of Myoferlin | 1.75 | 1.8 | 1.7 | |||
| 9 | IPI00021766 | RTN4 | Isoform 1 of Reticulon-4 | 1.55 | 1.6 | 1.5 | |||
| 10 | IPI00216694 | PLS3 | Plastin-3 | 1.55 | 1.6 | 1.5 | |||
| 11 | IPI00382844 | ACO2 | Aconitase (Fragment) | 1.55 | 1.5 | 1.6 | |||
| 12 | IPI00013860 | HIBADH | 3-hydroxyisobutyrate dehydrogenase, mitochondrial | 1.55 | 1.5 | 1.6 | |||
| 13 | IPI00018350 | MCM5 | DNA replication licensing factor MCM5 | 1.55 | 1.6 | 1.5 | |||
| 14 | IPI00098902 | OGDH | 2-oxoglutarate dehydrogenase, mitochondrial | 1.5 | 1.5 | 1.5 | |||
| 15 | IPI00784156 | AP2B1 | Isoform 1 of AP-2 complex subunit beta | 1.5 | 1.5 | 1.5 | |||
| 16 | IPI00456969 | DYNC1H1 | Cytoplasmic dynein 1 heavy chain 1 | 1.5 | 1.5 | 1.5 | |||
| Fraction (2 Gy × 5) | |||||||||
| 1 | IPI00022418 | FN1 | Isoform 1 of Fibronectin | 11.25 | 17.7 | 4.8 | |||
| 2 | IPI00011229 | CTSD | Cathepsin D | 3.35 | 3.4 | 3.3 | |||
| 3 | IPI00218474 | ENO3 | Isoform 1 of Beta-enolase | 2.8 | 4.6 | 1 | |||
| 4 | IPI00019157 | CSPG4 | Chondroitin sulfate proteoglycan 4 | 2.6 | 2 | 3.2 | |||
| 5 | IPI00005707 | MRC2 | C-type mannose receptor 2 | 2.45 | 2.5 | 2.4 | |||
| 6 | IPI00940829 | NEDD4 | Isoform 4 of E3 ubiquitin-protein ligase NEDD4 | 2.4 | 2.4 | 2.4 | |||
| 7 | IPI00020557 | LRP1 | Prolow-density lipoprotein receptor-related protein 1 | 2.2 | 1.8 | 2.6 | |||
| 8 | IPI00216457 | HIST2H2AA3 | Histone H2A type 2-A | 2.2 | 2.3 | 2.1 | |||
| 9 | IPI00020632 | ASS1 | Argininosuccinate synthase | 2.15 | 2.4 | 1.9 | |||
| 10 | IPI00026314 | GSN | Isoform 1 of Gelsolin | 1.95 | 2 | 1.9 | |||
| 11 | IPI00018398 | PSMC3 | 26S protease regulatory subunit 6A | 1.95 | 2.8 | 1.1 | |||
| 12 | IPI00021766 | RTN4 | Isoform 1 of Reticulon-4 | 1.9 | 1.7 | 2.1 | |||
| 13 | IPI00021048 | MYOF | Isoform 1 of Myoferlin | 1.85 | 2.4 | 1.3 | |||
| 14 | IPI00293464 | DDB1 | DNA damage-binding protein 1 | 1.75 | 1.4 | 2.1 | |||
| 15 | IPI00815770 | SNX3 | Isoform 1 of Sorting nexin-3 | 1.75 | 2.3 | 1.2 | |||
| 16 | IPI00024915 | PRDX5 | Isoform Mitochondrial of Peroxiredoxin-5, mitochondrial | 1.7 | 1.5 | 1.9 | |||
| 17 | IPI00007682 | ATP6V1A | V-type proton ATPase catalytic subunit A | 1.7 | 1.7 | 1.7 | |||
| 18 | IPI00021812 | AHNAK | Neuroblast differentiation-associated protein AHNAK | 1.6 | 1.6 | 1.6 | |||
| 19 | IPI00010418 | MYO1C | Isoform 2 of Myosin-Ic | 1.6 | 1.8 | 1.4 | |||
| 20 | IPI00000005 | NRAS | GTPase NRas | 1.6 | 1.6 | 1.6 | |||
| 21 | IPI00006482 | ATP1A1 | Isoform Long of Sodium/potassium-transporting ATPase subunit alpha-1 | 1.6 | 1.4 | 1.8 | |||
| 22 | IPI00023006 | ACTC1 | Actin, alpha cardiac muscle 1 | 1.6 | 2.1 | 1.1 | |||
| 23 | IPI00221035 | BTF3 | Isoform 1 of Transcription factor BTF3 | 1.6 | 1.6 | 1.6 | |||
| 24 | IPI00007402 | IPO7 | Importin-7 | 1.55 | 2.2 | 0.9 | |||
| 25 | IPI00246058 | PDCD6IP | Programmed cell death 6-interacting protein | 1.55 | 1.7 | 1.4 | |||
| 26 | IPI00026689 | CDK1 | Putative uncharacterized protein DKFZp686L20222 | 1.5 | 1.5 | 1.5 | |||
| 27 | IPI00001960 | CLIC4 | Chloride intracellular channel protein 4 | 1.5 | 1.4 | 1.6 | |||
Proteins increased by an average of 1.5-fold or more are listed.
*Statistical comparisons were performed using the paired z-test, and experimentally verified proteins are represented in bold. S: single-dose irradiation, F; fractionated-dose irradiation.