Table 3.
Metabolic parameters of mice fed the control diet (CD) or the high-fat diet (HFD)†
(Mean values with their standard errors)
| 2 weeks | 8 weeks | |||||||
|---|---|---|---|---|---|---|---|---|
| CD | HFD | CD | HFD | |||||
| Mean | sem | Mean | sem | Mean | sem | Mean | sem | |
| Body weight (g) | 24·8 | 0·5 | 27·5** | 0·3 | 26·2 | 0·7 | 32·0*** | 0·4 |
| Fed blood glucose (mmol/l) | 8·49 | 0·67 | 10·60 | 0·39 | 9·82 | 0·44 | 12·21** | 0·44 |
| Fasted blood glucose (mmol/l) | 4·77 | 0·17 | 7·49*** | 0·61 | 3·94 | 0·28 | 8·77** | 0·50 |
| Change in insulin (pg/ml) | 466 | 78 | 938 | 202 | 528 | 68 | 919* | 170 |
| Change in glucagon (pg/ml) | −24 | 5 | 6** | 5 | −23 | 3 | 5** | 7 |
| Total cholesterol (mmol/l) | 2·55 | 0·12 | 3·42** | 0·28 | ||||
| HDL (mmol/l) | 1·71 | 0·08 | 2·29* | 0·19 | ||||
| TAG (mmol/l) | 0·73 | 0·05 | 0·61 | 0·07 | ||||
| NEFA (mmol/l) | 0·55 | 0·06 | 0·49 | 0·06 | ||||
| Hydroxybutyrate (mmol/l) | 0·11 | 0·01 | 0·12 | 0·02 | ||||
Mean value was significantly different from that of the CD group: * P < 0·05, ** P < 0·01, *** P < 0·001 (non-parametric Mann–Whitney test).
† Body weight and fed blood glucose concentrations were measured weekly at 09.00 hours (n 8–16). Fasted blood glucose was measured after 16 h fasting. Changes in insulin and glucagon were calculated by the difference between glucose-stimulated minus basal plasma levels (n 8). Total and LDL-cholesterol, TAG, NEFA and hydroxybutyrate were measured in plasma of fasted mice after the oral glucose challenge (n 4).