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Effect of CDCA on ATP sensor (AT1.03YEMK) in AR42J cells. a Effect of 0.5 mM CDCA on FRET ratio of the ATP sensor in AR42J cells (n = 25). b Lifetime images of cells before and after treatment with CDCA. Images, where each pixel was analysed, are average of the two lifetime constants corrected for intensity scale. The insert table summarizes FLIM-FRET life constants T1 and T2 in 25 independent experiments. * P = 0.0001 for T1 and P = 0.007 for T1 and T2 comparison
