Fig. 7.

Effect of P2 receptor inhibitors on intracellular Ca²⁺ responses induced by CDCA in Capan-1 cells. a and b Representative recordings of [Ca²⁺]_i transients in Capan-1 cells with or without P2 receptors inhibitors (standing bath). Cell were incubated with a mix of P2R inhibitors: PPADS (250 μM), suramin (250 μM), and 10 μM of AZ 10606120 and 10 μM of A438079 for 25 min. a, c The presence of P2R antagonists markedly inhibited [Ca²⁺]_i response induced by ATP (100 μM), but had no effect on Thapsigargin (1 μM) induced Ca²⁺ response. b, d Incubation cells with P2R antagonists inhibited [Ca²⁺]_i transient induced by 0.3 mM CDCA but not by Thapsigargin (1 μM). c, d Change in [Ca²⁺]_i above baseline are given as mean values ± SEM of 7–15 cells per each independent experiment (n). Arrows indicate the time of adding the stimuli. *= P < 0.05, ***= P < 0.001, N.S = not significant