Table 2.
Sequences of the primers and probe used in this study
| Application | Primer name | Length(bp) | Primer/probe sequence(5′ to 3′) |
|---|---|---|---|
| Set 1 primers for RT-LAMP | M1-F3 | 20 | GGACACATTCTTGGTGGTCT |
| M1-B3 | 20 | CCAACACGTCCGTAGACAAT | |
| M1-FIP | 42 | TGGTGCTCCAAGCACTGGAATGACGCGCTTCTCACTACTTCT | |
| M1-BIP | 40 | AAGGTTGCTACTGGCGTACAGGTTGTAGTGGCCTTGGCGA | |
| Set 2 primers for RT-LAMP | M2-F3 | 18 | ACAGACGCGCTTCTCACT |
| M2-B3 | 20 | CCAACACGTCCGTAGACAAT | |
| M2-FIP | 39 | AGCGTTACACCAGTTGGTGCTCTTCTGTGATGGGCCGAC | |
| M2-BIP | 40 | AAGGTTGCTACTGGCGTACAGGTTGTAGTGGCCTTGGCGA | |
| Set 3 primers for RT-LAMP | M3-F3 | 19 | GCGCAGGACACATTCTTGG |
| M3-B3 | 19 | TTGGCGACTGTGACGAAAT | |
| M3-FIP | 40 | GGAATGCAGACCTGTCGGCCTCAATCCTGAAACAGACGCG | |
| M3-BIP | 41 | TGGAGCACCAACTGGTGTAACGGTACGCCAGTAGCAACCTT | |
| Set 4 primers for RT-LAMP | M4-F3 | 18 | CCGACAGGTCTGCATTCC |
| M4-B3 | 20 | CCAGTGCCAGATGAAGCATT | |
| M4-FIP | 42 | CCTGTACGCCAGTAGCAACCTTGAGCACCAACTGGTGTAACG | |
| M4-BIP | 41 | ATTTCGTCACAGTCGCCAAGGCGACTGAACGACCAACACGT | |
| Set 5 primers for RT-LAMP | M5-F3 | 20 | AGCTTTCAGGTCAATTGGGT |
| M5-B3 | 20 | GGAGTGTTAGCGTTACACCA | |
| M5-FIP | 42 | TGCGCCACAACCGAATGCTATTCAGCATCCTTATGGCTTGCA | |
| M5-BIP | 41 | TCAATCCTGAAACAGACGCGCTTGCTCCAAGCACTGGAATG | |
| RT-PCR | P1 | 21 | TTCCCAGCGTAGTTGAGATTG |
| P2 | 21 | CGAAGTGGCTCTGGATTTGTT | |
| real-time RT-PCR | PED-NF | 24 | CGCAAAGACTGAACCCACTAATTT |
| PED-NR | 24 | TTGCCTCTGTTGTTACTTGGAGAT | |
| PED-Cy5 | 24 | Cy5-TGTTGCCATTGCCACGACTCCTGC-BHQ3 |