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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Immunol. 2015 Feb 25;194(7):3011–3019. doi: 10.4049/jimmunol.1402650

Figure 2. IRBP-specific Tregs are highly enriched in inflamed eyes and have a demethylated TSDR.

Figure 2

B10.RIII FoxP3-GFP reporter mice were challenged for EAU. A) 17 days post immunization, cells from the eyes and draining peripheral LNs (draining the immunization site) were harvested and stained with CD4 and the IRBP161-180 MHC class II dimer and analyzed by flow cytometry. Shown is one representative experiment of five. B) 17 days post-immunization, eye cells were stained and sorted for the designated populations. Methylation status of Foxp3 TSDR was assessed per Materials and Methods. Shown is a combination of 2 experiments.