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. 2014 Nov 18;3(11):e210. doi: 10.1038/mtna.2014.61

Figure 4.

Figure 4

Effect of high polyethylene glycol (PEG) shielding on efficacy of FVII silencing after i.v. targeting of hepatocytes by ApoE- and ASGPR-mediated routes. (a) Dose-response of siRNA-mediated Factor VII silencing after i.v. delivery of lipid nanoparticles (LNP)1.5, LNP5, or LNP10. Serum Factor VII protein levels were measured in C57BL/6 mice (n = 4) 48 hours post-i.v. administration. (b) Silencing is abrogated in ApoE−/− mice (black bars). Surface addition of targeting ligand (GalNAc) to LNP1.5 (with 0.5% GalNAc-PEG-DSG) and LNP5 (with 0.5% GalNAc-PEG-DSG) restores silencing (light and dark grey bars). Serum Factor VII protein levels were measured in ApoE−/− mice (n = 3) 48 hours post-i.v. administration.