Skip to main content
. 2015 May 27;21:1527–1534. doi: 10.12659/MSM.894323

Figure 3.

Figure 3

miR-133 can directly target SLC39A1 and modulate its expression. (A, B) qRT-PCR and Western blot analysis of SLC39A1 expression in mMSCs derived from bone marrow of OVX mouse model (A) and hMSCs derived from bone marrow of PMOP (B). (C, D) qRT-PCR analysis of SLC39A1 expression in mMSCs derived from bone marrow of OVX mouse model (C) and hMSCs derived from bone marrow of PMOP (D) at different time points during osteogenic differentiation. (E) Predicted pairing and mutant sequence between SLC39A1 3′UTR and miR-133. (F) Western blot analysis of SLC39A1 expression in hMSCs with miR-133 overexpression or knockdown. (G) Dual luciferase assay of the relative luciferase activity in HEK-293T cells co-transfected with 150-ng reporter plasmids and 50-M miR-133 mimics. At 18 h after transfection, both firefly and Renilla luciferase activities were measured and the firefly luciferase activity was normalized to the Renilla luciferase activity. Data are shown as mean ±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001, NS P>0.5.