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. 2015 May 27;21:1527–1534. doi: 10.12659/MSM.894323

Figure 4.

Figure 4

MiR-133 modulates osteogenic differentiation of MSCs through SLC39A1. (A) Western blot analysis of SLC39A1 expression in hMSCs transfected with pLenti-SLC39A1 (without 3′ UTR) or co-transfected with pLenti-SLC39A1 (without 3′ UTR) and miR-133 mimics. (B, C) ALP staining (B) and Alizarin red S staining (C) were performed to determine the osteogenic differentiation of hMSCs transfected with pLenti-SLC39A1 (without 3′ UTR) or co-transfected with pLenti-SLC39A1 (without 3′ UTR) and miR-133 mimics. (D, E) Western blot analysis of RUNX2 (D) and Osterix (E) expression in hMSCs transfected with pLenti-SLC39A1 (without 3′ UTR) or co-transfected with pLenti-SLC39A1 (without 3′ UTR) and miR-133 mimics. Data are shown as mean ±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001, NS P>0.5.