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. Author manuscript; available in PMC: 2015 Jun 8.
Published in final edited form as: Methods Mol Biol. 2014;1124:251–268. doi: 10.1007/978-1-62703-845-4_16

Fig. 3.

Fig. 3

Detecting upregulation of proteins at the cell surface. Neutrophils plated on fibrinogen-coated coverslips were left untreated or stimulated with 200 nM PMA, 1 μM fMLF, or 0.02 μM TNFα as indicated. Fixed, intact cells were stained with mAb 7D5 to show upregulation of flavocytochrome b558 at the cell surface. All four images were acquired using identical confocal settings