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Neutrophil modulation of the extracellular matrix. PMNs were plated on coverslips coated with Oregon Green-labeled fibrinogen and then left untreated or stimulated with PMA, TNFα, or fMLF as indicated. PMNs were counterstained with mAb to gp91phox after fixation and permeabilization. Fluorescence microscopy images show the ability of activated cells to bleach and/or degrade fibrinogen. Differential effects of these stimuli on cell migration are also apparent
