Fig. 5.

Localization of NADPH oxidase subunits. Neutrophils were plated on fibrinogen-coated coverslips and then stimulated with TNFα for 30 min. Fixed and permeabilized cells were double stained to detect p22^phox (green) and p67^phox (red). Confocal sections taken through the center of cells or at the substrate-adherent surface indicate colocalization of NADPH oxidase subunits at the leading edge and throughout the plasma membrane (arrows). In contrast, p67^phox was not detected on p22^phox -positive specific granules (arrowheads)