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. Author manuscript; available in PMC: 2015 Jun 8.
Published in final edited form as: Cell. 2002 Aug 23;110(4):479–488. doi: 10.1016/s0092-8674(02)00861-9

Figure 3. Prevention of Hypertrophy by MITR.

Figure 3

(A and B) Cardiomyocytes were cultured either in the absence of virus or were infected with adenoviruses encoding GFP or Myc-tagged MITR S218/448A. After stimulation with PE or FBS for 24 hr, cells were fixed and stained with antibody against sarcomeric α-actinin (A, red signal) or ANF (B, perinuclear red signal). Adenovirus-infected cells were identified by GFP fluorescence or costaining with anti-Myc antibody to reveal ectopic MITR (green signal). Scale bar equals 20 μm.

(C) Cardiomyocytes were cultured and infected with adenoviruses as described above. Cells maintained in serum-free medium were stimulated with FBS, PE, ET-1, ANGII, or IGF-1. After 24 hr, RNA was isolated and the indicated transcripts were measured by dot blot. Numbers indicate the expression level of each transcript in the presence of Ad-MITR S218/448A relative to Ad-GFP and normalized to GAPDH.