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. 2015 Jun 9;13:39. doi: 10.1186/s12951-015-0098-0

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Gene silencing efficiency in vitro. Silencing of GAPDH gene in CHO-K1 cells was evaluated by quantitative real time polymerase chain reaction (qRT-PCR). GAPDH siRNA concentration was 50 nM. Lipo was the positive control, and scrambled siRNA was used as the negative control. Results are expressed as mean ± standard deviation (n = 3). *P value <0.05, the gene silencing efficiency at a molar ratio of 40/1 in a serum free environment is significantly different from the other treatments.