Figure S3.

Global Epigenetic Dynamics in hPGCs, Related to Figure 3

(A–C) Immunofluorescence analysis for (A) UHRF1 and DNMT1; (B) DNMT3A and DNMT3B and (C) TET1 on human embryo cryosections. Arrowheads in (A) indicate examples of Ki-67-positive (proliferating) hPGCs which are UHRF1-negative. Conventional ESCs [4th row in (B)], which strongly expresses DNMT3B, is used as a positive control in contrast to the absence of DNMT3B signals in Wk7 F ridges. Scale bars, 20 μm.

(D) Expression of epigenetic modifiers in various human samples by RNA-Seq.

(E and F) Immunofluorescence analysis for (E) H3K9me2; (F) H3K9me3 and HP1α/ MacroH2A2 on human embryo cryosections. Scale bars, 20 μm.