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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Autoimmun. 2015 Apr 2;59:8–18. doi: 10.1016/j.jaut.2015.01.003

Fig. 4.

Fig. 4

WKY rats immunized with c-α3-Gly peptide developed antibodies directed towards α3(IV)NC1. (A) Sera from animals immunized with c-α3-Gly peptide contained circulating antibodies reactive with recombinant α3(IV)NC1 by capture ELISA (*p<0.001; c-α3-Gly versus scrambled). (B) Sera from animals immunized with pCol(24–38) and c-α3-Gly peptide were reactive with recombinant α3(IV)NC1 by Western blotting. (C) Coomassie stained gel of collagenase-solubilised rat glomerular basement membrane (GBM) NC1 collagen IV (kindly provided by Dr. Billy Hudson) showed a range of hexamer, dimer and monomer bands. (E) Sera from animals immunized with c-α3-Gly peptide bound to NC1 hexamers and α3 monomers from GBM, similar to that from the sense pCol(24–38) peptide immunized animals. Positive control sera from animals immunized with recombinant rat α3(IV)NC1, and negative control sera from pre-immune animals and from animals given adjuvant alone.