Skip to main content
. Author manuscript; available in PMC: 2015 Jun 8.
Published in final edited form as: Science. 2014 Jul 31;345(6201):1139–1145. doi: 10.1126/science.1254917

Fig. 2. Native or S-to-G mutated variants of SRSF2 localize to different nuclear puncta.

Fig. 2

GFP fusion proteins linked to either the native, full-length SRSF2 or the SRSF2G1, SRSF2G2 or SRSF2G1/G2 mutants were transfected in U2OS cells in the absence (A) or presence (B) of a co-expressed mCherry:CLK1 fusion protein. The native SRSF2 protein localized to nuclear speckles and was dispersed into the nucleoplasm in the presence of co-transfected mCherry:CLK1. The SRSF2G1 and SRSF2G2 mutants localized to nucleoli as deduced by co-staining with antibodies specific to the nucleolar marker, Fibrillian. The SRSF2G1 mutant was partially redistributed from nucleoli to the cytoplasm in the presence of mCherry:CLK1. The SRSFG2 mutant was partially redistributed from nucleoli to the nucleoplasm in the presence of mCherry:CLK1. Co-expression of mCherry:CLK1 had no effect on the nucleolar localization of the SRSF2G1/G2 mutant.