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. Author manuscript; available in PMC: 2015 Jun 8.
Published in final edited form as: Science. 2014 Jul 31;345(6201):1139–1145. doi: 10.1126/science.1254917

Fig. 3. Binding of translation products of C9ORF72 hexanucleotide repeat expansion to mCherry:hnRNPA2 hydrogel droplets.

Fig. 3

Recombinant fusion proteins linking GFP to 20 repeats of the SR, GR or PR polymers (GFP:SR20, GFP:GR20 or GFP:PR20) were applied to slide chambers containing mCherry:hnRNPA2 hydrogel droplets. After overnight incubation at 4°C, all three proteins were trapped to the periphery of the hydrogels droplets (top panels). When incubated with reaction mixtures containing either the CLK1 or CLK2 protein kinase enzymes, pre-bound GFP:SR20 was released from the hydrogels in an ATP-dependent manner. GFP:GR20 or GFP:PR20 pre-bound to mCherry:hnRNPA2 hydrogel droplets were immune to the release by CLK1 or CLK2, even in the presence of ATP.