qPCR | ||
β-actin | GCCCTGAGGCACTCTTCCA | TTGCGGATGTCCACGTCA |
E-cadherin | GTCCTGGGCAGAGTGAATTT | GACCAAGAAATGGATCTGTGG |
Vimentin | CGAGGAGAGCAGGATTTCTC | GGTATCAACCAGAGGGAGTGA |
ZEB1 | AAGAATTCACAGTGGAGAGAAGCCA | CGTTTCTTGCAGTTTGGGCATT |
Bisulfite sequencing | ||
E-cadherin promoter | TTTAGTAATTTTAGGTTAGAGGGTTAT | AAACTCACAAATACTTTACAATTCC |
E-cadherin exon 2 | GTTTTGTTATTTTGGTTTTGA | AAAACTTACCCATTACAACC |
miR-200c, 141 | AAGGTTATTAGGGGAGAGGTTT | CTTCAAACCCAAAATCCCTA |
miR-203 | GAATTCGGGAGGTTAGGTG | ACCCCCTACCCTACTACAACC |
ChIP | ||
E-cadherin exon 2 | GCCGAGAGCTACACGTTCA | CCAACCCCTCCCTACTCC |
miR-200c,141 | AGGGCTCACCAGGAAGTGT | AGATCCCTGGCTCCCATC |
miR-200b,a,429 | CAGCTCGGGCAGCCGTGG | GTCGCTGCGTGCAGGGCTC |
miR-203 | CGTCTAAGGCGTCCGGTA | GAGCTGCGGAGAGAGGAG |