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. 2015 Jun 8;10(6):e0129772. doi: 10.1371/journal.pone.0129772

Fig 1. ABCB10-V5 expression and detection in intact mitochondria and SMPs.

Fig 1

A) Representative Western blot analysis of over-expressed murine ABCB10-V5 in HEK293 cells after loading 20 ug of protein from total cell lysates, isolated mitochondria or cytosol in a reducing SDS-PAGE gel. ABCB10 (aprox. 65kD) was detected using anti-V5 antibody (1:5000). Detection of mitochondrial outer-membrane protein porin was used as a control for mitochondrial protein content. B) ABCB10-V5 expression in sub-mitochondrial particles (SMPs) obtained from isolated mitochondria from HEK293 cells transfected with empty vector (Control) or with ABCB10-V5 encoding construct. ABCB10 was detected by Western blot using anti-ABCB10 polyclonal antibody (1:2000, showing 3 unspecific bands) (left) and anti-V5 (1:5000) (right) antibody (shown by arrows).