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. 2015 Jun 8;10(6):e0129772. doi: 10.1371/journal.pone.0129772

Fig 5. d-Amino-levulinic acid (d-ALA) does not alter 8-azido-ATP-[γ]-biotin photolabeling in ABCB10, whereas GSH and GSSG do.

Fig 5

A) Increasing concentrations of delta-aminolevulinic acid (d-ALA) did not change the photolabeling of ABCB10 by 8-azido-ATP [γ] biotin. Representative Western blot analyses are shown. The bar graph shows the relative intensity of ExtrAvidin-HRP as mean ± SD of independent experiments. B) and C) Increasing concentrations of GSH or GSSG decreased the biotin signal during the photolabeling of ABCB10 by 8-azido-ATP [γ] biotin. The bar graph shows the relative ExtrAvidin-HRP signal. Three independent experiments were conducted and data are shown as mean ± SEM. *p<0.05 vs. signal in 0mM GSH or GSSG treatment.