Fig 6. 3D reconstruction of electron microscopy image showed immunogold labels of vGluT2 associated with large dense-core vesicles in a neuroterminal of a representative middle-aged VEH treated rat.

(A) Lower magnification electron microscopy image was taken from the portal capillary zone of the lateral median eminence. A vGluT2 immunolabeled neuroterminal (Term, pseudocolored in light orange) was separated from the portal capillary area (pseudocolored in pink) by tanycyte endfeet (Tan, pseudocolored in green). (B) An adjacent section showed the same vGluT2 immunopositive neuroterminal from panel A in higher magnification. The 10 nm immunogold labels for vGluT2 were seen on large dense-core vesicles (arrows). (C) The terminal shown in panel A and B was 3D reconstructed from 13 images taken from a serial section ribbon. Reconstruction showed this terminal was covered by tanycyte endfeet (green). The majority of the vesicles in this terminal were large dense-core vesicles (yellow). Immunogold beads labeled for vGluT2 were individually marked in black dots and showed extensive association to large dense-core vesicles. A large mitochondria (purple) was located in the center of the terminal. BL = basal lamina, Mit = mitochondria, Tan = Tanycyte, Term = Neuroterminal. Scale bar panel A = 100 μm, B = 500 nm, C = 200 nm.