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. 2015 Mar 19;19(6):1197–1207. doi: 10.1111/jcmm.12454

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of miR-218-1 transgenic mice in vivo. (A) Haematoxylin and eosin staining was used to detect the phenotype alternation in the rectum of miR-218-1 transgenic mice. Arrow in the left panel indicated the gangliocytes, the middle indicated the aganglionosis, while the right indicated the compensatory proliferation of the nerve fibres. (B and C) Immunohistochemistry assays were conducted to detect the expression of the biomarker of gangliocytes (PGP9.5 and cathepsin D). Arrow indicated positive staining of the proteins expressed in gangliocytes. (D and E) The mRNA and protein expression level of Ret and Plag1 were examined in miR-218-1 transgenic mice and wild-type mice. All experiments were performed in triplicate and presented as mean ± SE. * indicates significant difference compared with control group (P < 0.05). * indicates remarkable difference (P < 0.01).