Skip to main content
. 2015 Mar 27;19(6):1234–1244. doi: 10.1111/jcmm.12472

Figure 1.

Figure 1

Time-dependent effects of high glucose or S100B on Steap4 protein expression and Steap4 gene transcription in MES13 cells. Cells were exposed to normal glucose (NG, open bars), high glucose (HG, closed bars) or S100B (1 μM, closed bars). Steap4 or S100B protein expression was measured by immunoblotting and was normalized to that of α-tubulin. Steap4 gene transcription was measured by transient transfection of the porcine Steap4 promoter plasmid p-1402. (A) Time-dependent (24–72 hrs) effects of HG (left panel) or S100B (right panel) on Steap4 protein expression. (B) Effects of transient transfection of S100B siRNA on HG-induced Steap4 protein expression at 48 hrs. (C) Time-dependent (24–72 hrs) effects of HG (left panel) or S100B (right panel) on Steap4 gene transcription. (D) Effects of transient transfection of S100B siRNA on HG-induced Steap4 gene transcription at 48 hrs. Data were expressed as the means ± SEM of three independent experiments. *: P < 0.05 versus open bar. #: P < 0.05 versus HG alone.