Figure 2.

Time-dependent effects of high glucose on cytosolic and cell membrane Steap4 protein expression in MES13 cells. Cells were exposed to high glucose (27.5 mM) for 24–72 hrs. Cells were pelleted and the cytosolic and membrane protein were fractionated by the CNMCS compartmental protein extraction kit. GAPDH was used as a cytosolic marker whereas pan-cadherin was used as a cell membrane marker. Expression of Steap4 protein was measured by immunoblotting and was normalized to that of GAPDH in the cytosol or normalized to that of pan-cadherin in the cell membrane.