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. 2015 Mar 27;19(6):1234–1244. doi: 10.1111/jcmm.12472

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Time-dependent effects of high glucose on protein-protein interaction among RAGE, S100B and Steap4. Cells were exposed to high glucose (27.5 mM) for 24–72 hrs. (A) Immunoprecipitated (IP) S100B or Steap4 was immunoblotted (IB) with RAGE and electrophoresed. Solid arrow: RAGE, broken arrows: molecular weight (MW) markers. (B) Immunoprecipitated (IP) S100B was immunoblotted (IB) with Steap4 and electrophoresed. Solid arrow: Steap4, broken arrows: MW markers.