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. 2015 Mar 27;19(6):1234–1244. doi: 10.1111/jcmm.12472

Figure 4.

Figure 4

JNK, PI3K and JAK2-STAT3 are required for S100B-induced Steap4 protein expression and gene transcription. Cells were exposed to S100B (1 μM) for 48 hrs. (A) Steap4 protein expression was measured by immunoblotting and was normalized to that of α-tubulin. SB203580 (a p38 kinase inhibitor, 10 μM), PD98059 (an ERK1/2 inhibitor, 10 μM), SP600125 (a JNK inhibitor, 10 μM), SB431542 (a type I TGF-β receptor inhibitor, 10 μM). LY294002 (a PI3K inhibitor, 10 μM) or AG490 (a JAK2 inhibitor, 10 μM) was administered. (B) Steap4 gene transcription was measured by transient transfection of the porcine Steap4 promoter plasmid p-1402. (C) The role of STAT3 in S100B (1 μM)-induced Steap4 protein expression was tested by using the wild-type (WT) and the serially mutated Steap4 promoter constructs (Mut S1, Mut S2, Mut S3, Mut S1.S2, Mut S1.S3, Mut S2.S3 and Mut S1.S2.S3). Data were expressed as the means ± SEM of three independent experiments. *: P < 0.05 versus open bars. #: P < 0.05 versus lane 2.