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. 2015 Mar 27;19(6):1234–1244. doi: 10.1111/jcmm.12472

Figure 5.

Figure 5

Effects of overexpressing Steap4 on high glucose or S100B-induced Steap4, collagen IV, fibronectin and COX2 protein expression. Cells were exposed to normal glucose (NG, open bars) or high glucose (HG, closed bars) or S100B (1 μM, closed bars) for 48 hrs. Protein expression of collagen IV (col4α1), fibronectin and COX2 was measured by immunoblotting and was normalized to that of α-tubulin. Steap4 overexpression was achieved by transient transfection of the pCMV-SPORT6-Steap4 expression plasmid (2 μg). (A) Effects of overexpressing Steap4 on HG-induced Steap4, collagen IV and fibronectin protein expression. (B) Effects of overexpressing Steap4 on S100B (1 μM)-induced Steap4, collagen IV and fibronectin protein expression. (C) Effects of overexpressing Steap4 on HG-induced COX2 protein expression. (D) Effects of overexpressing Steap4 on S100B (1 μM)-induced COX2 protein expression. Data were expressed as the means ± SEM of three independent experiments. *: P < 0.05 versus lane 1. #: P < 0.05 versus lane 2.