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. 2015 Mar 27;19(6):1234–1244. doi: 10.1111/jcmm.12472

Figure 7.

Figure 7

Effects of overexpressing Steap4 on S100B-induced signalling in cells and effects of overexpressing Steap4 on Steap4 protein expression in diabetic mice. Cells were exposed to S100B (1 μM) for 2 hrs. Steap4 overexpression was achieved by transient transfection of the pCMV-SPORT6-Steap4 expression plasmid. (A) Protein expression of Steap4, p-ERK1/2, ERK1/2, p-p38, p38, p-Akt, Akt, p-STAT3, STAT3, p-Smad2/3 and Smad2/3 was measured by immunoblotting and was normalized to that of α-tubulin. (B) Densitometry of (A). *: P < 0.05 versus lane 1. #: P < 0.05 versus lane 2. (C) Renal cortex was removed on week 8, homogenized and extracted protein was immunoblotted for Steap4 in control, diabetic (DM), vector-treated (DM + vector) and Steap4 plasmid-treated (DM + Steap4) mice. *: P < 0.05 versus lane 2. Data were expressed as the means ± SEM of three independent experiments.